Hello, Could you please help me with the sketcher?
I'm trying to use ccp4 sketcher to generate a new ligand and then complex it with a protein in coot. I've drawn the ligand, numbered each atom and defined each bond type. Then I ran save file, create library description. The pdb file was loaded to coot and worked fine. Then I imported cif file. However, when I tried to refine the ligand, a message popped out, saying 'No restrains'. I double checked the numbering, I think it's Ok. Did I do anything wrong? I'm really struggling on this. If you need more information, please let me know. Thanks very much. Best regards, Yahui
