Hi, I also have a question concerning anisotropic data. Collected a data set and the best crystal gave highly anisotropic diffraction patterns ( 3.7 A - 5.8 A). So my first question is how to handle these data. I got only experience with "normal data" using the ccp4 suite. Are there any program specially for these kind of data? There are? The second question is how anisotropic data occur? The protein I work with has a tetragonal sg with a=b= 86.0 and an extremely long c axis of 651 A. Secondary Structure prediction suggest a lot of beta strands. How can I explain the anisotropy (for my own interest and my thesis)? Thank you very much. Marie
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