I guess then Fred's reply part one explains everything :-) Jürgen
- Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/<http://web.me.com/bosch_lab/> On Jun 9, 2010, at 10:48 AM, Marie Lacroix wrote: Yes, I just checked this. ________________________________ Von: "Bosch, Juergen" <[email protected]<mailto:[email protected]>> An: Marie Lacroix <[email protected]<mailto:[email protected]>> Gesendet: Mittwoch, den 9. Juni 2010, 16:01:56 Uhr Betreff: Re: AW: AW: [ccp4bb] Anisotropic data and an extremely long c axis Does this coincide with the direction of better diffraction ? Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/<http://web.me.com/bosch_lab/> On Jun 9, 2010, at 9:38 AM, Marie Lacroix wrote: The crystal dimensions are 275 x 150 x 100 µm. So in one dimension they are bigger. ________________________________ Von: "Bosch, Juergen" <[email protected]<mailto:[email protected]>> An: Marie Lacroix <[email protected]<mailto:[email protected]>> Gesendet: Mittwoch, den 9. Juni 2010, 15:24:51 Uhr Betreff: Re: AW: [ccp4bb] Anisotropic data and an extremely long c axis but thicker in one dimension ? Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/<http://web.me.com/bosch_lab/> On Jun 9, 2010, at 8:41 AM, Marie Lacroix wrote: No, they are really large and beautiful 3D crystals. ________________________________ Von: Jürgen Bosch <[email protected]<mailto:[email protected]>> An: Marie Lacroix <[email protected]<mailto:[email protected]>> Gesendet: Mittwoch, den 9. Juni 2010, 14:38:37 Uhr Betreff: Re: [ccp4bb] Anisotropic data and an extremely long c axis Flat crystals ? Is the high resolution diffraction because you are shooting through more crystal volume ? Just a thought Jürgen ...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Jun 9, 2010, at 8:22, Marie Lacroix <[email protected]<mailto:[email protected]>> wrote: Hi, I also have a question concerning anisotropic data. Collected a data set and the best crystal gave highly anisotropic diffraction patterns ( 3.7 A - 5.8 A). So my first question is how to handle these data. I got only experience with "normal data" using the ccp4 suite. Are there any program specially for these kind of data? There are? The second question is how anisotropic data occur? The protein I work with has a tetragonal sg with a=b= 86.0 and an extremely long c axis of 651 A. Secondary Structure prediction suggest a lot of beta strands. How can I explain the anisotropy (for my own interest and my thesis)? Thank you very much. Marie
