Jerry McCully,
35% dioxane should be sufficient depending on the other components in your
buffer.
Yet, it is always advisable to try a few alternatives:
Try an extra 5-10% glycerol or ethylene glycol.
This might reduce the increase in mosaicity due to cooling shock.
If what you are crystallizing is a water soluble protein, you should take
a look at:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2253454/?tool=pubmed
High concentrations of organic solvents (MPD, DMSO, dioxane) are not
always the best thing to use
to crystallize proteins.
If this concerns you, consider starting from the conditions you
have now and try seeding into more conventional crystallization
conditions. Dioxane may just be
needed for a simple crystal contact and you may not require as much.
see:
http://pubs.acs.org/cgi-bin/article.cgi/cgdefu/2007/7/i11/html/cg700698d.html
Enrico.
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: [email protected] Fax: 33 (0)1 69 08 90 71
