And if you are not so into reading papers, you can use this database http://idb.exst.jaxa.jp/db_data/protein/search-e.php?
Jürgen P.S. Who wants to write an App for that, wouldn't this be very handy at the beamline ? I take 5% of the income for the idea :-) - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Oct 21, 2010, at 1:07 PM, Edward Snell wrote: > Not to forget > > J. Appl. Cryst. (1996). 29, 584-587 [ doi:10.1107/S0021889896004190 ] > Glycerol concentrations required for cryoprotection of 50 typical protein > crystallization solutions > E. F. Garman and E. P. Mitchell > > Which prompted the McFerrin and Snell work. > > Also worth checking out (and I apologize if I missed others) > > Acta Cryst. (2008). D64, 287-301 [ doi:10.1107/S0907444907067613 ] > Glycerol concentrations required for the successful vitrification of cocktail > conditions in a high-throughput crystallization screen > R. Kempkes, E. Stofko, K. Lam and E. H. Snell > > J. Appl. Cryst. (2006). 39, 244-251 [ doi:10.1107/S0021889806004717 ] > Effects of cryoprotectant concentration and cooling rate on vitrification of > aqueous solutions > V. Berejnov, N. S. Husseini, O. A. Alsaied and R. E. Thorne > > > Cheers, > > Eddie > > Edward Snell Ph.D. > Assistant Prof. Department of Structural Biology, SUNY Buffalo, > Senior Scientist, Hauptman-Woodward Medical Research Institute > 700 Ellicott Street, Buffalo, NY 14203-1102 > Phone: (716) 898 8631 Fax: (716) 898 8660 > Skype: eddie.snell Email: [email protected] > Telepathy: 42.2 GHz > > Heisenberg was probably here! > > > -----Original Message----- > From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Eric > Larson > Sent: Thursday, October 21, 2010 12:14 PM > To: [email protected] > Subject: Re: [ccp4bb] **Possible spam**cryoprotetant for 35% Dioxane > > Hi Jerry, > > A great reference for initial cryocondition searches for many "standard" > crystallization solutions is the tables in: > > J. Appl. Cryst. (2002). 35, 538-545 [ doi:10.1107/S0021889802009238 ] > The development and application of a method to quantify the quality of > cryoprotectant solutions using standard area-detector X-ray images > M. B. McFerrin and E. H. Snell > > On the bottom of page 542 it says for 35% dioxane as the precipitant in > Hampton Crystal Screen II condition # 4: > > 25% glycerol, 25% PEG 400, 20% ethylene glycol, 15% propylene glycol > (1,2-propanediol) > > good luck, > > Eric > > __________________________ > Eric Larson, PhD > Biomolecular Structure Center > Department of Biochemistry > Box 357742 > University of Washington > Seattle, WA 98195 > > > > On Wed, 20 Oct 2010, Jerry McCully wrote: > > | Dear All; > | > | We just got some crystals from 35% (v/v) Dioxane. We are going to > collect some data soon. > | > | Does anyone have the experience with the cryoprotectant in this > condition? > | > | Thanks a lot, > | > | Jerry McCully > | > | > | > |
