Hi Jerry,
A great reference for initial cryocondition searches for many "standard"
crystallization solutions is the tables in:
J. Appl. Cryst. (2002). 35, 538-545 [ doi:10.1107/S0021889802009238 ]
The development and application of a method to quantify the quality of
cryoprotectant solutions using standard area-detector X-ray images
M. B. McFerrin and E. H. Snell
On the bottom of page 542 it says for 35% dioxane as the precipitant in Hampton
Crystal Screen II condition # 4:
25% glycerol, 25% PEG 400, 20% ethylene glycol, 15% propylene glycol
(1,2-propanediol)
good luck,
Eric
__________________________
Eric Larson, PhD
Biomolecular Structure Center
Department of Biochemistry
Box 357742
University of Washington
Seattle, WA 98195
On Wed, 20 Oct 2010, Jerry McCully wrote:
| Dear All;
|
| We just got some crystals from 35% (v/v) Dioxane. We are going to collect some data soon.
|
| Does anyone have the experience with the cryoprotectant in this condition?
|
| Thanks a lot,
|
| Jerry McCully
|
|
|
|
