It seems that the Fourier cat is up to no good again..
BR From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Hubing Lou Sent: Wednesday, November 24, 2010 6:09 AM To: [email protected] Subject: [ccp4bb] unusual diffraction spots Dear CCP4BBer, I recently collected a dataset at synchrotron. The diffraction was quite anisotropic with one direction to 2.1Angstrom while the other is 3.0Ang. What unusual is in the diffraction image (see the attached file), clearly at low resolution there were some spots with tails ("two ears") and at the high resolution shell the spots turned to be rod-shaped. Please, can anyone explain how this could be? Is this related to the anisotropy? The protein was N-terminal his6-tagged, we are currently preparing new samples with the His-tag removed. But any other suggestions are also very welcomed. Regards, Hubing
