Stefan Could the low angle reflections be from lamellar liquid crystals of the PEG (or a mixed PEG/water phase). Seems like you have 1st,2nd and 4th order reflections with presumably the 2nd order about 20A. I think 40A lamellar spacing is characteristic of some PEG liquid crystals. Colin
From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Stefan Münnich Sent: 06 April 2011 09:33 To: [email protected] Subject: [ccp4bb] diffraction of spherulites Hey guys, When I collect data from these spherulites/crystals (grown in 0.1 M sodium acetate, 0.1 M MOPS pH 7.5, 12 % (w/v) PEG-8000, protein buffer: 100 mM NaCl, 50 mM HEPES pH 7.5): http://img695.imageshack.us/i/cryst.png/ I get this diffraction pattern: (it's not cryo protected, so there's some ice-rings also) http://img683.imageshack.us/i/diffv.jpg/ It can't be only ice-rings because those are usually starting at something like 3.8 A, whereas I already got one ring directly around the beam center and also one at about 20 A. Has anybody seen anything like that and tell me what it is? Stefan
