Dear Pengfei,
Indeed, for "HDR crystallography" ("high dynamic range") simply (integrate and)
scale both batches of images at the same time in HKL2000. Depending on the
space group, make sure indexing is consistent between the two datasets. Scaling
then takes care of combining the two batches. Depending on how many overloads
you have in the high-exposure datasets you may have to adjust the resolution
ranges to avoid scaling problems. The two batches must have a sufficient number
of well-measured, identical HKLs between them to figure out the relative scale
factors.
Hope that helps.
Cheers,
MM
On May 30, 2011, at 2:13 PM, Pengfei Fang wrote:
Dear All,
I have two data sets from one crystal.
One had short exposures to keep the low resolution reflections from saturating.
The other had long exposure to get high resolution data.
I am wondering how two merge these two data sets into one file that I can use
for refinement.
The two sets already scaled by HKL2000. Now I have two .sca files.
Thanks a lot!
Pengfei Fang
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Mischa Machius, PhD
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Assoc. Professor, Dept. of Pharmacology
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