[ccp4bb] small lysozyme crystals?

[James Holton]

Does anyone out there have a protocol of growing HEWL crystals that are 
all 50-100 microns wide?  I gave this project to a summer student 
recently, thinking it would be easy, but it is turning out to be more 
difficult than I thought.  Keep getting sphereulites instead of small 
crystals.  Yes, I know you can smash a large lysozyme crystal with a 
hammer, but that is not exactly what I was going for.  What I was hoping 
for was a well-defined protocol for growing "reference" crystals that 
stay evenly illuminated in our x-ray beams as they rotate.  The beam is 
100 um wide.

I'm sure someone has done this before?
-James Holton
MAD Scientist