Hi all, I recently got diffraction data of 214 AA protein. When I processed the data, pointless suggested me a space group as P41. However, when I ran Phaser with 'all choices of alternate space group', it gave me a pdb file with P43. Additionally, phenix.xtriage suggested me P422 with twin laws (h,-k,-l). Anyway, it seems like P43 is right space group (I tried to process and refine all possible space groups, and based on R values P43 is most reasonable). At this point, I'd like to know more first why different programs suggested different space group, and which is the most reliable way to determine the space group. Also, what is the biggest difference between these space groups. In practice, which way is the best method to get a right space group other than trying all possible space groups as I just did.
Thanks in advance, Young-Jin