Hi all,
I have a homotetrameric coiled-coil domain sample with 45aa per each. While I store this sample at 4oC, the sample looks clear w/o any particles. But when I took out the sample to my bench at r.t, I can see there are precipitates (as stack of needle like particles) at the bottom of the tube after several hours. Interestingly, when I put it back into 4oC fridge, the precipitates disappeared and the solution turned into clear again. Does anyone have knowledge of such behavior of any protein? I appreciate any information related. Min-Kyu
