Could be one of those weird behaviors displayed by detergents where cloud point anomalously changes with temperature...
Artem On Mar 12, 2012 1:11 PM, "Min-Kyu Cho" <[email protected]> wrote: > I am using KPi buffer at pH 5.5, 100mM KCl, 2mM beta-mercaptoethanol, 0.02% > NaN3. > > Yes, I agree I should check CD melting curve to see temperature preference > of my protein. > > Min-Kyu > > | -----Original Message----- > | From: Kevin Jin [mailto:[email protected]] > | Sent: Monday, March 12, 2012 11:16 AM > | To: Min-Kyu Cho > | Cc: [email protected] > | Subject: Re: [ccp4bb] My protein precipitates at r.t and dissolves at 4 > | oC > | > | Which kind of buffer you use? If it is Tris, then temperature change > will > | cause pH change. > | > | Actually, this is a good way for crystallization. > | > | Kevin > | > | On Mon, Mar 12, 2012 at 9:02 AM, Min-Kyu Cho <[email protected]> > wrote: > | > Hi all, > | > > | > I have a homotetrameric coiled-coil domain sample with 45aa per each. > | > While I store this sample at 4oC, the sample looks clear w/o any > | > particles. But when I took out the sample to my bench at r.t, I can > | > see there are precipitates (as stack of needle like particles) at the > | > bottom of the tube after several hours. Interestingly, when I put it > | > back into 4oC fridge, the precipitates disappeared and the solution > | turned into clear again. > | > > | > Does anyone have knowledge of such behavior of any protein? I > | > appreciate any information related. > | > > | > Min-Kyu >
