I am using KPi buffer at pH 5.5, 100mM KCl, 2mM beta-mercaptoethanol, 0.02% NaN3.
Yes, I agree I should check CD melting curve to see temperature preference of my protein. Min-Kyu | -----Original Message----- | From: Kevin Jin [mailto:[email protected]] | Sent: Monday, March 12, 2012 11:16 AM | To: Min-Kyu Cho | Cc: [email protected] | Subject: Re: [ccp4bb] My protein precipitates at r.t and dissolves at 4 | oC | | Which kind of buffer you use? If it is Tris, then temperature change will | cause pH change. | | Actually, this is a good way for crystallization. | | Kevin | | On Mon, Mar 12, 2012 at 9:02 AM, Min-Kyu Cho <[email protected]> wrote: | > Hi all, | > | > I have a homotetrameric coiled-coil domain sample with 45aa per each. | > While I store this sample at 4oC, the sample looks clear w/o any | > particles. But when I took out the sample to my bench at r.t, I can | > see there are precipitates (as stack of needle like particles) at the | > bottom of the tube after several hours. Interestingly, when I put it | > back into 4oC fridge, the precipitates disappeared and the solution | turned into clear again. | > | > Does anyone have knowledge of such behavior of any protein? I | > appreciate any information related. | > | > Min-Kyu
