Ed: Thank you very much for your advice and inputs
regards Ros On Wed, Apr 18, 2012 at 8:44 AM, Ed Pozharski <[email protected]>wrote: > On Tue, 2012-04-17 at 17:49 -0400, Uma Ratu wrote: > > In order to have my target .pdb, I need to mutate the residues using > > coot? > > Others already recommended CHAINSAW to prepare the model. Note that > coot has a nice feature under Extensions->All molecule... called "[Post > MR] Fill partial residues...". By default, CHAINSAW will truncate > non-conserved residues to gamma atom, so you will end up with some > weird-looking tryptophans. See > > http://www.biop.ox.ac.uk/coot/doc/coot/Fill-Partial-Residues.html > > for details. > > IMPORTANT! You still have to inspect the model manually, adjust some > side chains, build alternate conformers, fix the backbone where needed, > fill gaps etc. But you already know this. > > Cheers, > > Ed. > > -- > Coot verendus est > >
