I think some have used anomalous signals since the 1930s-40s, e.g., Bijvoet!
JPK On Wed, Jun 6, 2012 at 10:23 AM, Ronald E Stenkamp <[email protected]> wrote: > There were a number of labs using anomalous dispersion for phasing 40 years > ago. The theory for using it dates from the 60s. And careful experimental > technique allowed the structure solution of several proteins before 1980 > using what would be labeled now as SIRAS. Ron > > > On Wed, 6 Jun 2012, Dyda wrote: > >>> I suspect that pure MIR (without anomalous) was always a fiction. I doubt >>> that anyone has ever used it. Heavy atoms always give >>> an anomalous signal >> >> >>> Phil >> >> >> I suspect that there was a time when the anomalous signal in data sets was >> fictional. >> Before the invent of flash freezing, systematic errors due to decay and >> the need >> of scaling together many derivative data sets collected on multiple >> crystals could render >> weak anomalous signal useless. Therefore MIR was needed. Also, current >> hardware/software >> produces much better reduced data, so weak signals can become useful. >> >> Fred >> >> >> [32m******************************************************************************* >> Fred Dyda, Ph.D. Phone:301-402-4496 >> Laboratory of Molecular Biology Fax: 301-496-0201 >> DHHS/NIH/NIDDK e-mail:[email protected] >> Bldg. 5. Room 303 >> Bethesda, MD 20892-0560 URGENT message e-mail: [email protected] >> Google maps coords: 39.000597, -77.102102 >> http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred >> >> ******************************************************************************* >> [m >> > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: [email protected] *******************************************
