Bijvoet - 1949 ! FF Dr Felix Frolow Professor of Structural Biology and Biotechnology Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor e-mail: [email protected] Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Jun 6, 2012, at 18:28 , Jacob Keller wrote: > I think some have used anomalous signals since the 1930s-40s, e.g., Bijvoet! > > JPK > > On Wed, Jun 6, 2012 at 10:23 AM, Ronald E Stenkamp > <[email protected]> wrote: >> There were a number of labs using anomalous dispersion for phasing 40 years >> ago. The theory for using it dates from the 60s. And careful experimental >> technique allowed the structure solution of several proteins before 1980 >> using what would be labeled now as SIRAS. Ron >> >> >> On Wed, 6 Jun 2012, Dyda wrote: >> >>>> I suspect that pure MIR (without anomalous) was always a fiction. I doubt >>>> that anyone has ever used it. Heavy atoms always give >>>> an anomalous signal >>> >>> >>>> Phil >>> >>> >>> I suspect that there was a time when the anomalous signal in data sets was >>> fictional. >>> Before the invent of flash freezing, systematic errors due to decay and >>> the need >>> of scaling together many derivative data sets collected on multiple >>> crystals could render >>> weak anomalous signal useless. Therefore MIR was needed. Also, current >>> hardware/software >>> produces much better reduced data, so weak signals can become useful. >>> >>> Fred >>> >>> >>> >>> [32m******************************************************************************* >>> Fred Dyda, Ph.D. Phone:301-402-4496 >>> Laboratory of Molecular Biology Fax: 301-496-0201 >>> DHHS/NIH/NIDDK e-mail:[email protected] >>> Bldg. 5. Room 303 >>> Bethesda, MD 20892-0560 URGENT message e-mail: [email protected] >>> Google maps coords: 39.000597, -77.102102 >>> http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred >>> >>> ******************************************************************************* >>> [m >>> >> > > > > -- > ******************************************* > Jacob Pearson Keller > Northwestern University > Medical Scientist Training Program > email: [email protected] > *******************************************
