Do you have crystals? Do they diffract? If so, to what resolution?
What resolution do you require to answer your biological question? F On Jun 12, 2012, at 7:46 PM, LISA <[email protected]> wrote: > Hi all, > > My work is to solve huge complex containing 4 different proteins and total > molecular weight is about 300 KD. I can purify the complex by co-expression > them in E.coli. This complex contains 8 protein A, 2 protein B and 1 protein > C and D. protein B and protein C have homology structures deposited in PDB > database. No homology structure available for protein A and D, which > contribute 60% of the whole molecular weight for the complex. > > Now I am trying to find a way to solve the phase of this complex. I am > thinking of use sad or mad with se-Met. There total 111 Met residues in > this complex. Is it possible to solve this complex by se-Met? Does someone > have experience to solve huge complex structure with se-met? It is also very > welcome for all the suggestion. Thank you. > > All the best, > > Lisa
