Dear All, I will be grateful to your suggestions about Iron-Sulfur cluster protein purification. My colleague has some problems with the purification and it seems that the iron-sulfur cluster might be deformed, and protein is aggregated. The purification is done under aerobic (normal condition), and is there some method to reintegrate iron sulfur cluster back into the protein molecule under these conditions. Can anybody suggest some literature, protocol or something that can improve the protein aggregation?
I look forward to hearing from you. Best wishes, Jan
