Jan, Do you express the protein with the *E. coli isc* iron-sulfur cluster synthetic operon? I found great success, see:
Daughtry, KD et. al. JACS 2012 http://pubs.acs.org/doi/full/10.1021/ja2111898 - Kelly Daughtry ******************************************************* Kelly Daughtry, Ph.D. Post-Doctoral Fellow, Raetz Lab Biochemistry Department Duke University Alex H. Sands, Jr. Building 303 Research Drive RM 250 Durham, NC 27710 P: 919-684-5178 ******************************************************* On Wed, Jul 11, 2012 at 6:22 AM, Jan Rashid Umar <[email protected]>wrote: > Dear All, > > I will be grateful to your suggestions about Iron-Sulfur cluster protein > purification. My colleague has some problems with the purification and it > seems that the iron-sulfur cluster might be deformed, and protein is > aggregated. The purification is done under aerobic (normal condition), and > is there some method to reintegrate iron sulfur cluster back into the > protein molecule under these conditions. Can anybody suggest some > literature, protocol or something that can improve the protein aggregation? > > I look forward to hearing from you. > > Best wishes, > > Jan >
