Hi Jan, I wonder if the protein has a hexaHis tag. I recently was working on a Fe-S containing protein and noticed significant aggregation/precipitation. After I cleaving the His tag, the enzyme seems stable for days in the same buffer. HTH, Yuri
- [ccp4bb] FE-Sulfur proteins Jan Rashid Umar
- Re: [ccp4bb] FE-Sulfur proteins Kelly Daughtry
- Re: [ccp4bb] FE-Sulfur proteins Edward A. Berry
- Re: [ccp4bb] FE-Sulfur proteins Kelly Daughtry
- Re: [ccp4bb] FE-Sulfur proteins Colbert, Christopher
- Re: [ccp4bb] FE-Sulfur proteins Bosch, Juergen
- Re: [ccp4bb] FE-Sulfur proteins Yuri Pompeu
