Dear Appu,

I had similar issue once. You can use Coot to overlay two structures and
get the rotation matrix

a1 a2 a3
b1 b2 b3
c1 c2 c3

from here you can calculate cosine of an angle using formula

(a1+b2+c3-1)/2

Good luck,

Nikolina Sekulic, PhD
postdoctoral fellow
University of Pennsylvania

On Thu, Aug 23, 2012 at 2:30 PM, Appu kumar <[email protected]> wrote:

> Dear all ccp4 users,
>                                   I have solved the structure of a protein
> which is a dimer. On comparison with the other structure from PDB data
> bank, i found that structure solved by us show a tiltness in
> monomer-monomer arrangement of a dimer. May you please help me in findng
> the angle between two monomer. Do i need to calculate the centre of mass of
> the protein for calcualting the angle between them?.
> Thanks in advance please help me.
> Thank you
> Appu
>

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