Sir one more help plz i have the following matrix which one shall i use to calculate the angle.
INFO:: coordinates transformed by orthonal matrix: | -0.6927, -0.7191, 0.0564| | -0.4828, 0.5204, 0.7043| | -0.5358, 0.4606, -0.7076| ( 76.04, -10.19, 28.64) Rotation - polar (omega,phi,kappa) 69.7525 112.368 160.045 Rotation - euler (alpha,beta,gamma) 85.4221 135.041 40.6865 Translation - Angstroms 76.037 -10.1858 28.6373 If you won't mind please tell me how to calculathe angle from this. I am new to this type of problem. Thank you Appu On 24 August 2012 08:52, Appu kumar <appu.kum...@gmail.com> wrote: > Thank you Sir for your kind eply. I will follow your instruction to > calculate the angle. > > > > On 24 August 2012 00:38, Nikolina Sekulic <nikolina.seku...@gmail.com>wrote: > >> Dear Appu, >> >> I had similar issue once. You can use Coot to overlay two structures and >> get the rotation matrix >> >> a1 a2 a3 >> b1 b2 b3 >> c1 c2 c3 >> >> from here you can calculate cosine of an angle using formula >> >> (a1+b2+c3-1)/2 >> >> Good luck, >> >> Nikolina Sekulic, PhD >> postdoctoral fellow >> University of Pennsylvania >> >> >> On Thu, Aug 23, 2012 at 2:30 PM, Appu kumar <appu.kum...@gmail.com>wrote: >> >>> Dear all ccp4 users, >>> I have solved the structure of a >>> protein which is a dimer. On comparison with the other structure from PDB >>> data bank, i found that structure solved by us show a tiltness in >>> monomer-monomer arrangement of a dimer. May you please help me in findng >>> the angle between two monomer. Do i need to calculate the centre of mass of >>> the protein for calcualting the angle between them?. >>> Thanks in advance please help me. >>> Thank you >>> Appu >>> >> >> >
