the angle of rotation is kappa = 160.045deg in this case On 24 Aug 2012, at 04:33, Appu kumar wrote:
> Sir one more help plz i have the following matrix which one shall i use to > calculate the angle. > > INFO:: coordinates transformed by orthonal matrix: > | -0.6927, -0.7191, 0.0564| > | -0.4828, 0.5204, 0.7043| > | -0.5358, 0.4606, -0.7076| > ( 76.04, -10.19, 28.64) > Rotation - polar (omega,phi,kappa) 69.7525 112.368 160.045 > Rotation - euler (alpha,beta,gamma) 85.4221 135.041 40.6865 > Translation - Angstroms 76.037 -10.1858 28.6373 > > If you won't mind please tell me how to calculathe angle from this. I am new > to this type of problem. > Thank you > Appu > > > > > > On 24 August 2012 08:52, Appu kumar <[email protected]> wrote: > Thank you Sir for your kind eply. I will follow your instruction to calculate > the angle. > > > > On 24 August 2012 00:38, Nikolina Sekulic <[email protected]> wrote: > Dear Appu, > > I had similar issue once. You can use Coot to overlay two structures and get > the rotation matrix > > a1 a2 a3 > b1 b2 b3 > c1 c2 c3 > > from here you can calculate cosine of an angle using formula > > (a1+b2+c3-1)/2 > > Good luck, > > Nikolina Sekulic, PhD > postdoctoral fellow > University of Pennsylvania > > > On Thu, Aug 23, 2012 at 2:30 PM, Appu kumar <[email protected]> wrote: > Dear all ccp4 users, > I have solved the structure of a protein > which is a dimer. On comparison with the other structure from PDB data bank, > i found that structure solved by us show a tiltness in monomer-monomer > arrangement of a dimer. May you please help me in findng the angle between > two monomer. Do i need to calculate the centre of mass of the protein for > calcualting the angle between them?. > Thanks in advance please help me. > Thank you > Appu > > >
