Dear George,
Le 13/11/12 11:17, George a écrit :
Dear colleagues,
There are some crystallographers with (much) more experience than me.
I ‘ve attached few diffraction images which are not (in my opinion)
typical salt but not typical protein either.
Please let me know you suggestions. Is it worth investigating further
those conditions or there are just salts crystals with large unit cell.
Attached files:
Crystal.jpg (Photo of crystal)
dif_0.jpg (Diffraction 900 sec exposure/degree at 0 degrees)
dif_90.jpg (Diffraction 900 sec exposure/degree at 90 degrees)
dif_180.jpg (Diffraction 900 sec exposure/degree at 180 degrees)
dif_270.jpg (Diffraction 900 sec exposure/degree at 270 degrees)
protein: 3.3mg/ml in 20 mM TrisHCl pH 8, 150 mM NaCl, 5 mM DTT.
Mother liquor: 28%Jefamine, 0,05mM Li2SO4, 10mM Tris pH 8.0
sitting drops, 1.2ul protein / 1.2ul mother liquor.
Thanks in advance for your help,
George Kontopidis
