-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear George,
the images don't look like a large cell to me: on the first image you can see spots from ice rings at about 4A and there are only very few spots inside that radius, all of which are at beyond 5A. I'd say there are traces of MgS04 or CaSO4, the Ca or Mg being left-overs from the expression media. Regards, Timd On 11/13/2012 11:17 AM, George wrote: > > > Dear colleagues, > > > > There are some crystallographers with (much) more experience than > me. > > I ‘ve attached few diffraction images which are not (in my opinion) > typical salt but not typical protein either. > > Please let me know you suggestions. Is it worth investigating > further those conditions or there are just salts crystals with > large unit cell. > > Attached files: > > Crystal.jpg (Photo of crystal) > > dif_0.jpg (Diffraction 900 sec exposure/degree at 0 degrees) > > > dif_90.jpg (Diffraction 900 sec exposure/degree at 90 degrees) > > > dif_180.jpg (Diffraction 900 sec exposure/degree at 180 degrees) > > > dif_270.jpg (Diffraction 900 sec exposure/degree at 270 degrees) > > > > > > > protein: 3.3mg/ml in 20 mM TrisHCl pH 8, 150 mM NaCl, 5 mM DTT. > > Mother liquor: 28%Jefamine, 0,05mM Li2SO4, 10mM Tris pH 8.0 > > sitting drops, 1.2ul protein / 1.2ul mother liquor. > > > > Thanks in advance for your help, > > > > George Kontopidis > > > > From: CCP4 bulletin board [mailto:[email protected]] On Behalf > Of Frank von Delft Sent: Tuesday, November 13, 2012 8:56 AM To: > [email protected] Subject: Re: [ccp4bb] Reservoir buffer > > > > > > Acta Cryst. (2005). D61, 490-493 [ doi:10.1107/S0907444905002726 > <http://dx.doi.org/10.1107/S0907444905002726> ] > > > Expanding screening space through the use of alternative reservoirs > in vapor-diffusion experiments > > > J. Newman > <http://scripts.iucr.org/cgi-bin/citedin?search_on=name&author_name=Newman,%20J.> > > > > Abstract: Setting up vapor-diffusion crystallization experiments > against four different reservoir solutions showed that the > reservoir solution may have a profound effect on the outcome of a > crystallization experiment. This suggests that a facile way to > increase crystallization space through screening is not to add more > crystallization conditions to the process, but to set up the same > conditions over different reservoirs. > > > > > > On 13/11/2012 06:03, Theresa Hsu wrote: > > Dear all > > In *initial screening* using vapor diffusion crystallization, does > it matter whether the reservoir buffer is also the precipitant in > the drop or just a high salt solution like 5 M NaCl? > > Thank you. > > Theresa > > > > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFQoiIZUxlJ7aRr7hoRAlOtAKD9vm5OgG/GH/SMXv4LwTybKnlU3gCghuVH dFhZk5C5gK3fjVG1z00+jzw= =VN4A -----END PGP SIGNATURE-----
