Ganesh!!! NO WAY !!! FF Dr Felix Frolow Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor e-mail: [email protected] Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Nov 13, 2012, at 12:40 , Ganesh Natrajan <[email protected]> wrote: > Hi, > > First I'm sorry for my blank message earlier. > > Doesn't this depend on the oscillation angle? If those images were collected > using 0 to 1° oscillations, I would assume he has a badly diffracting protein > crystal. > > Ganesh > > > > Le 13/11/12 11:34, Tim Gruene a écrit : >> -----BEGIN PGP SIGNED MESSAGE----- >> Hash: SHA1 >> >> Dear George, >> >> the images don't look like a large cell to me: on the first image you >> can see spots from ice rings at about 4A and there are only very few >> spots inside that radius, all of which are at beyond 5A. >> I'd say there are traces of MgS04 or CaSO4, the Ca or Mg being >> left-overs from the expression media. >> >> Regards, >> Timd >> >> On 11/13/2012 11:17 AM, George wrote: >>> >>> Dear colleagues, >>> >>> >>> >>> There are some crystallographers with (much) more experience than >>> me. >>> >>> I ‘ve attached few diffraction images which are not (in my opinion) >>> typical salt but not typical protein either. >>> >>> Please let me know you suggestions. Is it worth investigating >>> further those conditions or there are just salts crystals with >>> large unit cell. >>> >>> Attached files: >>> >>> Crystal.jpg (Photo of crystal) >>> >>> dif_0.jpg (Diffraction 900 sec exposure/degree at 0 degrees) >>> >>> >>> dif_90.jpg (Diffraction 900 sec exposure/degree at 90 degrees) >>> >>> >>> dif_180.jpg (Diffraction 900 sec exposure/degree at 180 degrees) >>> >>> >>> dif_270.jpg (Diffraction 900 sec exposure/degree at 270 degrees) >>> >>> >>> >>> >>> >>> >>> protein: 3.3mg/ml in 20 mM TrisHCl pH 8, 150 mM NaCl, 5 mM DTT. >>> >>> Mother liquor: 28%Jefamine, 0,05mM Li2SO4, 10mM Tris pH 8.0 >>> >>> sitting drops, 1.2ul protein / 1.2ul mother liquor. >>> >>> >>> >>> Thanks in advance for your help, >>> >>> >>> >>> George Kontopidis >>> >>> >>> >>> From: CCP4 bulletin board [mailto:[email protected]] On Behalf >>> Of Frank von Delft Sent: Tuesday, November 13, 2012 8:56 AM To: >>> [email protected] Subject: Re: [ccp4bb] Reservoir buffer >>> >>> >>> >>> >>> >>> Acta Cryst. (2005). D61, 490-493 [ doi:10.1107/S0907444905002726 >>> <http://dx.doi.org/10.1107/S0907444905002726> ] >>> >>> >>> Expanding screening space through the use of alternative reservoirs >>> in vapor-diffusion experiments >>> >>> >>> J. Newman >>> <http://scripts.iucr.org/cgi-bin/citedin?search_on=name&author_name=Newman,%20J.> >>> >>> >>> >>> Abstract: Setting up vapor-diffusion crystallization experiments >>> against four different reservoir solutions showed that the >>> reservoir solution may have a profound effect on the outcome of a >>> crystallization experiment. This suggests that a facile way to >>> increase crystallization space through screening is not to add more >>> crystallization conditions to the process, but to set up the same >>> conditions over different reservoirs. >>> >>> >>> >>> >>> >>> On 13/11/2012 06:03, Theresa Hsu wrote: >>> >>> Dear all >>> >>> In *initial screening* using vapor diffusion crystallization, does >>> it matter whether the reservoir buffer is also the precipitant in >>> the drop or just a high salt solution like 5 M NaCl? >>> >>> Thank you. >>> >>> Theresa >>> >>> >>> >>> >> - -- - -- >> Dr Tim Gruene >> Institut fuer anorganische Chemie >> Tammannstr. 4 >> D-37077 Goettingen >> >> GPG Key ID = A46BEE1A >> >> -----BEGIN PGP SIGNATURE----- >> Version: GnuPG v1.4.12 (GNU/Linux) >> Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ >> >> iD8DBQFQoiIZUxlJ7aRr7hoRAlOtAKD9vm5OgG/GH/SMXv4LwTybKnlU3gCghuVH >> dFhZk5C5gK3fjVG1z00+jzw= >> =VN4A >> -----END PGP SIGNATURE----- >>
