Of course, common in baculo:
http://dx.doi.org/10.1038/emboj.2009.352
The EMBO Journal (2010) 29,505--514
*How baculovirus polyhedra fit square pegs into round holes to robustly
package viruses*
Xiaoyun Ji, Geoff Sutton, Gwyndaf Evans, Danny Axford, Robin Owen and
David I Stuart
On 15/02/2013 20:00, Savvas Savvides wrote:
Hi Jacob,
check out Figure 1 in
Natively inhibited Trypanosoma brucei cathepsin B structure determined by using
an X-ray laser.
Redecke L, et al.
Science. 2013 Jan 11;339(6116):227-30.
and
In vivo protein crystallization opens new routes in structural biology.
Koopmann R. Nature Methods. 2012 Jan 29;9(3):259-62.
best
Savvas
On 15 Feb 2013, at 20:44, Jacob Keller wrote:
Dear Crystallographers,
I was looking at some live, control HEK cells expressing just eGFP, and to my
great surprise, saw littered across the dish what appeared to be small
fluorescent needles (see attached--sorry about the size, but it's only ~1MB
total.) Can these possibly be fortuitous protein crystals? They were too small
to mount I think, and for what it's worth, parallel-transfected HeLa cells did
not have these things. But, some needles could be seen in the DIC images as
well, and the needles were only fluorescent with GFP filter sets, and not CFP,
YFP, or texas red filters. I thought of whale myoglobin crystallizing on the
decks of ships, but never thought I would see this....
Jacob
--
*******************************************
Jacob Pearson Keller, PhD
Postdoctoral Associate
HHMI Janelia Farms Research Campus
email: [email protected]
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<GFP_crystals_DIC.png><GFP_crystals_Fluorescence.png>
