Dear All, Thanks for the help and suggestion
Francis, we used molrep for the structure solution. As I told that we already have the structure of the same protein, but a variant. So, we used its single chain (heteromer) as a model for molecular replacement. and Eleanor, I deleted once that chain and did refinement, all I was getting was the small small broken electron density everywhere in that chain region. Thanks again. -- Sonali Dhindwal “Live as if you were to die tomorrow. Learn as if you were to live forever.” ________________________________ From: Francis E. Reyes <[email protected]> To: sonali dhindwal <[email protected]> Sent: Monday, 29 April 2013 10:03 PM Subject: Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit Where are the phases coming from? MR? F On Apr 28, 2013, at 10:02 PM, sonali dhindwal <[email protected]> wrote: > Dear All, > > We are working on a crystal structure with 12 molecules in an asymmetric > unit. It is a heteromer and each chain is made up of two type of chains. > Therefore there are 24 chains in an asymmetric unit. > > It is giving solution in P1. Rfactor and Rfree has reached 20.25 and 25.6 at a resolution of 1.8Angstrom > > All the chains are refining well and have good electron density except 3 > chains which have very poor electron density in most of the region. > > Whereas, the crystal structure of variants of the same protein we have solved > earlier refined well and has very good electron density in all the chains. > > What could be the possible reason for these three chains which are not > refining properly. Any suggestions will be very useful. > > Thanks and Regards > > -- > Sonali Dhindwal > > “Live as if you were to die tomorrow. Learn as if you were to live forever.” --------------------------------------------- Francis E. Reyes PhD 215 UCB University of Colorado at Boulder
