and 2VAK...average Bs for the twelve, sequence identical, chains vary from 28 to 53.
On 29 Apr 2013, at 22:09, David Schuller wrote: > On 04/29/13 12:26, Roger Rowlett wrote: >> FYI, I do know of one example of a solved structure where some of the >> molecules in the ASU are poorly defined. In 1EKJ, 4 of the 8 molecules in >> the ASU have low B-factors (mid 30s) and 4 have high B-factors (50s-60s). In >> the unit cell these layers alternate. It is possible, if everything else has >> been excluded, that your crystal has a similar crystal-packing issue. > > Another is 3HDH. > Pig short chain L-3-hydroxyacyl-CoA dehydrongenase revisited: sequence > analysis and crystal structure determination. > Barycki, et al (1999) Protein Science 8: 2010-2018. > > "Examination of the map in the region of subunit C revealed that > the electron density was considerably weaker for the third subunit > compared to the other subunits..." > -- > ======================================================================= > All Things Serve the Beam > ======================================================================= > David J. Schuller > modern man in a post-modern world > MacCHESS, Cornell University > schul...@cornell.edu
