Dear All,
Thanks for the reply.
I will try process and refine the data again, will see if it improves.
Thanks again to all.
--
Sonali Dhindwal
“Live as if you were to die tomorrow. Learn as if you were to live forever.”
________________________________
From: Mark van Raaij <mjvanra...@cnb.csic.es>
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, 30 April 2013 1:42 AM
Subject: Re: [ccp4bb] Poor electron density in some of the chains in an
asymmetric unit
and 2VAK...average Bs for the twelve, sequence identical, chains vary from 28
to 53.
On 29 Apr 2013, at 22:09, David Schuller wrote:
On 04/29/13 12:26, Roger Rowlett wrote:
>
>FYI, I do know of one example of a solved structure where some of the
>molecules in the ASU are poorly defined. In 1EKJ, 4 of the 8 molecules in the
>ASU have low B-factors (mid 30s) and 4 have high B-factors (50s-60s). In the
>unit cell these layers alternate. It is possible, if everything else has been
>excluded, that your crystal has a similar crystal-packing issue.
>>
>Another is 3HDH.
>Pig short chain L-3-hydroxyacyl-CoA dehydrongenase revisited:
sequence analysis and crystal structure determination.
>Barycki, et al (1999) Protein Science 8: 2010-2018.
>
>
>"Examination of the map in the region of subunit C revealed that
>the electron density was considerably weaker for the third subunit
>compared to the other subunits..."
>
>--
=======================================================================
All Things Serve the Beam
======================================================================= David
J. Schuller modern man in a post-modern world MacCHESS, Cornell University
schul...@cornell.edu
