Hi Alan, Is there a specific reason to choose Saccharomyces? I know a lot of labs use Pichia for protein production. I think you might look into it.
On Mon, Aug 5, 2013 at 5:55 AM, Alan F Scott <[email protected]>wrote: > Dear All, > > Sorry for the off-topic post. I am looking to overproduce a protein for > crystallography purposes in *Saccharomyces cerevisiae*. Nobody in my lab > has ever overproduced a protein in yeast before so I need to try producing > a control protein first. Does anybody know an *E. coli *or yeast protein > which can be easily produced in *Saccharomyces *in large quantities > please? Thanks for any suggestions. > > Best wishes, > > Alan >
