Taking Tfz of 14.3 I assume that you have used Phaser, and according to what authors say, it is most probably the solution. Maybe map is not yet good, but you can try to refine using modern approaches for refinement of low resolution structures which are recently implemented in Refmac and Phenix. Your map will look better after refinement, as model can move quite significant distance into better position. Phase extension into better resolution data is a method which is developed already the late 80's. So you are in… Good luck FF Dr Felix Frolow Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor e-mail: [email protected] Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Sep 24, 2013, at 24:01 , RHYS GRINTER <[email protected]> wrote: > Hi all, > > I have been attempting to find a MR solution for a low resolution data set > (3.9A), with pretty poor merging stats of a 22 strand membrane beta barrel > I'm working on. > > I've created a trimmed poly-alanine from a structure of 17% identity, that > gives a solution with a Tfz of 14.3 with two molecules per asu (llg is around > 900). I'm guessing this in a genuine solution, but the map is too poor to > build into. > > Does anyone have any advice as to proceed from here? It may be just a case of > needing better resolution data to work with, but would this indicate that > Selenomet derivative crystals won't be needed for this structure? > > Cheers, > > Rhys
