>From my experience, my two cents: 1) Retain as much of the structure that fits the density well (no need to trim to poly ala completely), remove the rest and keep refining and adding on. You seem to have got the right solution. 2) Try simulated annealing and omit map calculations to improve on the map. 3) Re-look the data for twinning, pseudo-symmetry or other signs of problem in data.
Good luck! Anita On Tue, Sep 24, 2013 at 2:31 AM, RHYS GRINTER < [email protected]> wrote: > Hi all, > > I have been attempting to find a MR solution for a low resolution data set > (3.9A), with pretty poor merging stats of a 22 strand membrane beta barrel > I'm working on. > > I've created a trimmed poly-alanine from a structure of 17% identity, that > gives a solution with a Tfz of 14.3 with two molecules per asu (llg is > around 900). I'm guessing this in a genuine solution, but the map is too > poor to build into. > > Does anyone have any advice as to proceed from here? It may be just a case > of needing better resolution data to work with, but would this indicate > that Selenomet derivative crystals won't be needed for this structure? > > Cheers, > > Rhys
