Dear Krithika,
what you probably see is the diffraction pattern of a highly mosaic
crystal due to probably internal disordering.
cheers
Demetres
On 3/10/2013 3:40 μμ, Krithika GOkulnath wrote:
I introduce myself as Dr.Krithika Gokulnath, CAS in crystallography and
Biophysics, UNiversity of Madras. I have been working with a cloned protein and
have been successfully able to crystallise it. However data collection done at
home source shows a pattern of streaks instead of spots. The crystallization
set up contained 12% PEG 8000, 10mM Magnesium chloride, 200mM Potassium
chloride in 50 mM Tris pH 7.0. The protein is an enzyme which is also in the
buffer containing the above components excepting the precipitant. It is a
tetramer at native conditions and molecular weight on SDS-PAGE is 54 Kda.
Kindly share your suggestions and speculations on what might be the reason for
the streaking. The streaks are many wide spread and there are no high
resolution spots or streaks as we might expect in salt crystals.
--
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Dr. Demetres D. Leonidas
Associate Professor of Biochemistry
Department of Biochemistry & Biotechnology
University of Thessaly
26 Ploutonos Str.
41221 Larissa, Greece
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Tel. +302410 565278
Tel. +302410 565297 (Lab)
Fax. +302410 565290
E-mail: [email protected]
http://www.bio.uth.gr
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