One standard thing to do would be to set up a grid around the condition and then try picking crystals from different well to see if any of them produce better looking diffraction patterns. If that does not work one might give any of the commercially available additive screen a try to see if diffraction improves.
On Thu, Oct 3, 2013 at 6:32 PM, Demetres D. Leonidas <[email protected]>wrote: > Dear Krithika, > > what you probably see is the diffraction pattern of a highly mosaic > crystal due to probably internal disordering. > > cheers > > Demetres > > > > On 3/10/2013 3:40 μμ, Krithika GOkulnath wrote: > >> I introduce myself as Dr.Krithika Gokulnath, CAS in crystallography and >> Biophysics, UNiversity of Madras. I have been working with a cloned protein >> and have been successfully able to crystallise it. However data collection >> done at home source shows a pattern of streaks instead of spots. The >> crystallization set up contained 12% PEG 8000, 10mM Magnesium chloride, >> 200mM Potassium chloride in 50 mM Tris pH 7.0. The protein is an enzyme >> which is also in the buffer containing the above components excepting the >> precipitant. It is a tetramer at native conditions and molecular weight on >> SDS-PAGE is 54 Kda. Kindly share your suggestions and speculations on what >> might be the reason for the streaking. The streaks are many wide spread and >> there are no high resolution spots or streaks as we might expect in salt >> crystals. >> > > -- > ------------------------------**----------------- > Dr. Demetres D. Leonidas > Associate Professor of Biochemistry > Department of Biochemistry & Biotechnology > University of Thessaly > 26 Ploutonos Str. > 41221 Larissa, Greece > ------------------------------**--------------- > Tel. +302410 565278 > Tel. +302410 565297 (Lab) > Fax. +302410 565290 > E-mail: [email protected] > http://www.bio.uth.gr > ------------------------------**----------------- > -- -- स्वस्तिक फुलेरा वरिष्ठ अनुसंधान कर्ता संरचनात्मक जीवविज्ञान प्रयोगशाला डी. एन. ए. फिंगरप्रिंटिंग एवं निदान केंद्र हैदराबाद, ५००, ००१ और राष्ट्रीय कोशिका विज्ञान केंद्र पुणे विश्वविद्यालय परिसर, गणेशखिंड पुना, ४११,००७
