On 20 Nov 2013, at 21:56, Dhanasekaran Varudharasu wrote:

> Dear Crystallographers,
> 
>                                         I dialysed a 30 kDa protein 
> (Recombinant protein which was eluted by 20 mM Tris, 500 mM NaCl, 120 mM 
> imidazole) against water for overnight. But it gets precipitated after 12  
> hours. Can anybody give some suggestion to avoid precipitation.  


... dont dialyse it against water!

A.


> Thanks
> Dhana 
> 
> 
> 
> 

Reply via email to