-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Hello Dhana,
use a buffer at least, and some salt - if I remember correctly, gel filtration resins require 150mM salt concentration for proper separation (might have been 50mM). Even 150mM may be too little to keep your protein soluble. Best, Tim On 11/20/2013 09:56 PM, Dhanasekaran Varudharasu wrote: > Dear Crystallographers, > > I dialysed a 30 kDa protein (Recombinant protein which was eluted > by 20 mM Tris, 500 mM NaCl, 120 mM imidazole) against water for > overnight. But it gets precipitated after 12 hours. Can anybody > give some suggestion to avoid precipitation. Thanks Dhana > - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.15 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFSjSt9UxlJ7aRr7hoRAhkCAKD1AoxzFIRYkKTGl6gqNv27h1wPgQCff/4I 500w4xBATx9AtInbHgJgeCQ= =YU/J -----END PGP SIGNATURE-----
