Hi
do not forget the clusters like Ta6Br12 or the lanthanides.
in case your interesting protein is a membrane protein there are some
choices that might work better than other
we have described it here.
http://www.ncbi.nlm.nih.gov/pubmed/16855303
This is not exclusive to membrane proteins at all. My personal favorite
is orange platinum, with an acupunture needle stick the tip in the
orange platinum powder, and and gently let the needle hoover over the
drop, the static electricity will pull some orange platinum crystals
into your drop without disturbing it. This is really nice if you have
mechanically sensitive crystals. Then just seal the drop and wait for
the orange color to emerge. Its almost like cooking a restaurant dish,
just more expensive.
cheers
Preben
On 1/15/14 6:18 PM, RHYS GRINTER wrote:
Hello message board,
My group has some crystals of an interesting protein to take to the synchrotron
in a couple of weeks. We won't be able to prepare and crystallise a SelMet
derivative during that time period, but we have loads of crystals sitting
around. The diffraction isn't great, we see maybe 3.5 at home but might be
enough to get over the line.
It will be a very difficult MR target, so we were thinking of soaking so
crystals with heavy atomic compounds that we have lying around. I was wondering
if people had any suggestions of compounds that people have used successfully
for experimental phasing and maybe concentrations to use and soaking time.
Cheers,
Rhys
--
J. Preben Morth, Ph.D
Group Leader
Membrane Transport Group
Nordic EMBL Partnership
Centre for Molecular Medicine Norway (NCMM)
University of Oslo
P.O.Box 1137 Blindern
0318 Oslo, Norway
Tel: +47 2284 0794
