Keith's favourites over many years (shared with Gideon): K2PtCl4
K2PtCN4 KAu(CN)2 Ethyl Mercury Thiosalicylate (gentle Hg reagent) Try for about 2-12 hours at 1 mM initially. If crystals crack, reduce concentration - at least they bind! If these don't work, then finding a derivative is probably going to be hard1 K On 15 Jan 2014, at 17:18, RHYS GRINTER wrote: > Hello message board, > > My group has some crystals of an interesting protein to take to the > synchrotron in a couple of weeks. We won't be able to prepare and crystallise > a SelMet derivative during that time period, but we have loads of crystals > sitting around. The diffraction isn't great, we see maybe 3.5 at home but > might be enough to get over the line. > It will be a very difficult MR target, so we were thinking of soaking so > crystals with heavy atomic compounds that we have lying around. I was > wondering if people had any suggestions of compounds that people have used > successfully for experimental phasing and maybe concentrations to use and > soaking time. > > Cheers, > > Rhys keith.wil...@york.ac.uk ************************************************* Prof. Keith S. Wilson, Structural Biology Laboratory, University of York, YORK YO10 5DD UK tel 44-1904 328262 fax 44-1904 328266 ************************************************** Email disclaimer This email and its attachments may be confidential and are intended solely for the use of the intended recipient. If you are not the intended recipient of this email and its attachments, you must take no action based upon them, nor must you copy or show them to anyone. Please contact the sender if you believe you have received this email in error. Any views or opinions expressed are solely those of the author and do not necessarily represent those of The University of York.
