Hi,
You probably tried it but what happens if you fit in PMSF and refine?
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: [email protected]
Phone: 972-8-647-2220 Skype: boaz.shaanan
Fax: 972-8-647-2992 or 972-8-646-1710
________________________________________
From: CCP4 bulletin board [[email protected]] on behalf of Annemarie Weber
[[email protected]]
Sent: Monday, February 03, 2014 6:37 PM
To: [email protected]
Subject: [ccp4bb] unexplained density
Dear all,
I am refining a 1.4 A resolution structure and found some well-defined
but unfortunately unexplained density. The protein was purified in HEPES
buffer with PMSF and protease inhibitor cocktail tablets (Roche) added.
It was cocrystallized with AMPPNP in PEG2000MME and Tris. I modelled a
molecule into the density, which fits quite well but I do not know what
it is and how it got there. Attached are two pictures with the
difference density and the molecule I modelled in there.
Has anybody seen something like this before? Maybe some degradation
product of the buffer/PMSF?
Any suggestions will be highly welcome.
Thanks a lot
Annemarie
