It looks like a Hepes? Padayatti
On Mon, Feb 3, 2014 at 8:37 AM, Annemarie Weber < [email protected]> wrote: > Dear all, > I am refining a 1.4 A resolution structure and found some well-defined but > unfortunately unexplained density. The protein was purified in HEPES buffer > with PMSF and protease inhibitor cocktail tablets (Roche) added. It was > cocrystallized with AMPPNP in PEG2000MME and Tris. I modelled a molecule > into the density, which fits quite well but I do not know what it is and > how it got there. Attached are two pictures with the difference density and > the molecule I modelled in there. > Has anybody seen something like this before? Maybe some degradation > product of the buffer/PMSF? > Any suggestions will be highly welcome. > Thanks a lot > Annemarie > -- P
