Dear All
On similar lines any suggestions and tips on expression and
purification of the proteins containing both FAD and NADPH would be really
helpful.
I recently tried to express and purify a protein containing these two
ligands, but failed (aggregation of protein).
my initial questions are:
does addition of Riboflavin in the media has affect on protein expression?
considering reconstitution, I am concerned that NADHP will be oxidized
during processing.
what are the other alternatives for incorporating ligands, if
reconstitution doesn't help?
