Hello Peter,
1、THE RESOLUTION OF THE DATA IS 2.5 angstrom. After first refinement of > refmac5 I got R factor which is 0.26 and R FREE which is 0.31. My question > is what the final R factor and R FREE should be after several rounds of > refinement by refmac5 and coot. > answer can be found here: Acta Cryst. D65, 297-300 (2009) Direct link: http://phenix-online.org/papers/lv5003_reprint.pdf 2、At which map level（e/A3 or rmsd）should I refine the data by coot? > I guess you meant "refine model parameters".. just a remark. We normally refine atomic (and non-atomic) model parameters against measured data using some agreement criteria and a minimization method.. etc etc, you know all this! As to your actual question: if you hope for a meaningful answer, at least you need to tell what map you use, otherwise you'll get some spam. Pavel
