Dear Felix,

I recently had the same problem. I didn't think TER records were a big deal. I 
never had a program that did weird thing because of missing TER records. But 
well, orders are orders, so we need TER records for deposition. It would be 
nice if all refinement programs would write those. AFAIK, Phenix already does.

Rather then putting them in manually, I ended up using pdb_extract to convert 
my pdb file to mmCIF. A nice bonus is that you can throw in the log files from 
indexing and phasing which saves a lot of typing later in the deposition 
process.

Cheers,
Robbie

Sent from my Windows Phone
________________________________
Van: Felix Frolow
Verzonden: 13-5-2014 23:26
Aan: [email protected]
Onderwerp: [ccp4bb] TER in PDB file

Community!
I am in the process of PDB deposition.
I have used for refinement Refmac.
I enter to refinement cycle PDB file with TER separators.
After refinement they disappear.
PDB validation server (option 1 with geometrical parameters report ) DEMAND TER 
separator.
In my structure there are 28 hetero-mers.
Is it sane:
 To demand such thing?
Not to supply  such thing?
Do I miss something ?
Does community punch in TER cards manually ?

FF

Dr Felix Frolow
Professor of Structural Biology and Biotechnology, Department of Molecular 
Microbiology and Biotechnology
Tel Aviv University 69978, Israel

Acta Crystallographica F, co-editor

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