Dear Faisal, at 2.6A resolution I would not call 23A^2 and 24A^2 'a difference', rather a fluctuation. Parameters are not totally independent from each other and not from experimental issues. It most likely also make a difference which program you use. Some program has a upper cut-off of 100-200 (which I think is reasonable) while I have seen atoms in the PDB with B-values in the region of 500A^2 (which is close to saying the resolution of the data set is less than 5A...).
Best, Tim On 08/14/2014 10:30 AM, Faisal Tarique wrote: > Dear all > > I request you to please tell me the difference between wilson B-factor and > average B, all atoms ?? I have two structures one native at 2A resolution, > having mean b factor of 24 and the other a complex structure of the same > protein with a ligand (at 2.6A resolution) has mean b factor of 23..Why > there is a difference in the b factor for these two coordinates with the > later having (low resolution) lower value than the previous (high > resolution)..?? is this a normal phenomenon or the stability is due to > bound ligand molecule, which is stabilizing the whole structure ?? Please > pour some light in this direction.. > -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
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