Dear all,
I’m doing molecular replacement using Phaser. My protein is predicted
to have two domain with a “hinge” linking them. The model sequence identity is
0.27. But the MR result is poor. I’ve tried other programme (Molrep, MrBump,
Balbes,,,_.) But no improvement was observed. I think that this is due to the
“open” or “closed” conformation around the hinge. I was told that I could place
the Z axis along the hinge
(http://xray0.princeton.edu/~phil/Facility/Guides/MolecularReplacement.html),
could anyone tell me more details about how to do next?
Thanks!
Lu Zuokun
