If your Pt position is "centric" then either hand will give a reasonable looking solution but one will generate LH helices and the other right.
Model building can break that ambiguity - SHELXE and Buccaneer both will impose correct peptide geometry. But at such low resolution they may struggle.. but it is worth a try Eleanor On 16 December 2014 at 09:39, RHYS GRINTER <[email protected]> wrote: > > Hi All, > > This will no doubt show something of my ignorance with experimental > phasing, however I'm currently working on solving a 3.8A SAD dataset with > Pt anomalous signal. Both Shelx and Xtriage see reasonable anomalous signal > to about 7A and seem to get statistics suggesting a solution when I run > SAD phasing using Autosol in Phenix. After density modification I get > pretty nice looking maps with clear solvent channels and interconnected > density for protein, however there is very little difference in map > R-factor between the hands and the maps from both the P31 and P32 solution > appear comparable in structure. > Obviously DM is struggling to break the hand ambiguity, however can some > one tell me if what I'm seeing represents a definite solution? And what is > the best way to proceed from here? > > Cheers, > > Rhys
