Sorry - of course it can.. Sorry. Only if the twinning is perfect then you apparently get a higher symmetry.. Eleanor
On 16 December 2014 at 14:26, Tim Gruene <[email protected]> wrote: > > Dear Rhys, > > I would try to place idealised secondary structure elements with coot > into the density - at this resolution they probably fit both hands, but > you may see a difference when you do e.g. rigid body refinement. > > Best, > Tim > > On 12/16/2014 10:39 AM, RHYS GRINTER wrote: > > Hi All, > > > > This will no doubt show something of my ignorance with experimental > phasing, however I'm currently working on solving a 3.8A SAD dataset with > Pt anomalous signal. Both Shelx and Xtriage see reasonable anomalous signal > to about 7A and seem to get statistics suggesting a solution when I run > SAD phasing using Autosol in Phenix. After density modification I get > pretty nice looking maps with clear solvent channels and interconnected > density for protein, however there is very little difference in map > R-factor between the hands and the maps from both the P31 and P32 solution > appear comparable in structure. > > Obviously DM is struggling to break the hand ambiguity, however can some > one tell me if what I'm seeing represents a definite solution? And what is > the best way to proceed from here? > > > > Cheers, > > > > Rhys > > > > -- > Dr Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A > >
