Hi, Is Autobuster a online server? On 13-Jan-2015 2:27 pm, "Atul Kumar" <[email protected]> wrote:
> Dear Rohit, > > You can follow the way Tim suggested. At low resolution and for disordered > regions, autobuster works well for refinement. > > Atul > > On Tue, Jan 13, 2015 at 7:47 AM, Tim Gruene <[email protected]> > wrote: > >> Dear Rohit Kumar Singh, >> >> you can remove a couple of residues before and after the outlier and >> rebuild it. You can also switch on Ramachandran restraints in Coot and >> run real-space refinement. If this does not help, I would delete a >> single residue within the region in question, run real-space refinement >> for both segments and reconnect. >> >> Regards, >> Tim >> >> On 01/13/2015 08:35 AM, rohit kumar wrote: >> > Hello all, >> > >> > if outlier is in between 5 to 6 %, how someone can fix it. >> > As the resolution is low (3.0-3.5 A). >> > >> > On Mon, Jan 12, 2015 at 7:53 PM, Robbie P. Joosten <[email protected]> >> wrote: >> > >> >> Hi Dialing, >> >> >> >> 86% favoured can actually be quite okay for an initial model. As long >> as >> >> it doesn't have 14% in the disallowed region, the vast majority of >> residues >> >> will be correct, or close enough to the correct answer that they can be >> >> fixed easily. Of course, such a model will need tweaking, but this is >> more >> >> or less the point of calling it an 'initial' model. >> >> >> >> Cheers, >> >> Robbie >> >> >> >> >> >> On 01/12/2015 01:18 PM, Dialing Pretty wrote: >> >> >> >>> Dear All， >> >>> >> >>> If an initial PDB has only 86% residues in the Ramachandran favored >> >>> region, it >> >>> would mean there is a significant error (for example significant >> length of >> >>> protein fragment in the total protein assigned to the wrong electron >> >>> density map >> >>> position) , right? >> >>> >> >>> Dialing >> >>> >> >>> >> > >> > >> >> -- >> Dr Tim Gruene >> Institut fuer anorganische Chemie >> Tammannstr. 4 >> D-37077 Goettingen >> >> GPG Key ID = A46BEE1A >> >> >
